Abstract: Objective :To investigate the relation between the lung cancer and the EBV in pharyngeal secretion, blood and tumor tissue of lung cancer patients. Methods :PCR and ELISA methods was used detect EBV in pharygeal secretion, fresh lung cancer tissue and blood specimen taken from30 patients with lung cancer in progressing period. Results :EBV infection rate of pharyngeal secretion was low, and there was no difference between the patients with lung cancer and with nonumorous chest disease. EBV VCA-IgG antibody of lung cancer patients was superior that of nonumorous chest disease patients. EBV infection rate of lung cancer tissue was 43.3%, and there was no difference among various kinds of pathogen with EBV infection. There was a medium correlation between EBV infection in lung cancer tissue and blood EBV VCA-IgG antibody. Conclusions :EBV in lung cancer pa-tients blood and tumor tissue have been increasing. There is a close relation between lung cancer and long-term EBV infection.